normal colonic epithelial cell lines ccd 841 con  (ATCC)

Journal: Food Science & Nutrition

Article Title: Pigmented Sorghum Phenolic Extracts Regulate the Expression of Cancer Development Pathway Genes in HT ‐29 and Hypoxia‐Induced CCD 841 CoN Cells

doi: 10.1002/fsn3.71614

Figure Lengend Snippet: Changes in the RT‐PCR gene expression profile of cellular metabolism genes (HIF‐1α, HIF‐1β, and GLUT‐1) following raw BlackSs [HIF‐1α (A), HIF‐1β (B), and GLUT‐1 (C)] and BlackSb [HIF‐1α (D), HIF‐1β (E), and GLUT‐1 (F)] extract treatment on hypoxia‐induced CCD 841 CoN colon cells. Data is presented as mean ± SD ( n = 3). Statistical significance was determined by two‐way ANOVA with Dunnett's post hoc test, adjusting p values for Type I error when comparing multiple treatment groups to a single control. Significance level increases with decreasing p value represented by * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. HIF‐1α, hypoxia inducible factor 1 alpha; HIF‐1β, hypoxia inducible factor 1 beta; GLUT‐1, glucose transporter 1.

Article Snippet: HT‐29 colorectal cancer cells and normal CCD 841 CoN colon cells were sourced from the ATCC distributor In Vitro Technologies Pty Ltd. (Victoria, Australia).

Techniques: Reverse Transcription Polymerase Chain Reaction, Gene Expression, Control